童年的一起突发事件,令彼得·帕克(安德鲁·加菲尔德 Andrew Garfield 饰)和父母生离死别。转眼彼得成为一名高中生,他偶然发现父亲的公文包,并从叔父本(马丁·辛 Martin Sheen 饰)处得到线索,于是启程前往奥斯库公司拜访父亲当年的合作伙伴——科特·康纳斯博士(瑞斯·伊凡斯 Rhys Ifans 饰)。在公司内四处寻找线索的彼得意外被蜘蛛咬了一口,回程途中他的身体发生奇异的变化。在康纳斯博士德引导下,彼得不断了解并发掘体内着超乎寻常的能力,喜怒哀乐,各种意想不到的事情迅速向他袭来。直到某日,他便成了影响深远的超级战士蜘蛛侠。 而城市内也出现了凶恶的蜥蜴人,彼得不平凡的人生就此展开……Peter Parker (Garfield) is an outcast high schooler who was abandoned by his parents as a boy, leaving him to be raised by his Uncle Ben (Sheen) and Aunt May (Field). Like most teenagers, Peter is trying to figure out who he is and how he got to be the person he is today. Peter is also finding his way with his first high school crush, Gwen Stacy (Stone), and together, they struggle with love, commitment, and secrets. As Peter discovers a mysterious briefcase that belonged to his father, he begins a quest to understand his parents' disappearance - leading him directly to Oscorp and the lab of Dr. Curt Connors (Ifans), his father's former partner. As Spider-Man is set on a collision course with Connors' alter-ego, The Lizard, Peter will make life-altering choices to use his powers and shape his destiny to become a hero.